腺相關(guān)病毒（AAV）是非致病性ssDNA病毒，需要輔助病毒(通常為腺病毒)參與復(fù)制。它編碼兩個末端的反向重復(fù)序列(ITR)中的cap和rep基因。ITRs對于病毒的復(fù)制和包裝具有作用。cap基因編碼病毒衣殼蛋白，rep基因參與病毒的復(fù)制和整合。
重組腺相關(guān)病毒載體(rAAV) 源于非致病的野生型腺相關(guān)病毒，由于其安全性好、宿主細(xì)胞范圍廣(分裂和非分裂細(xì)胞)、免疫源性低，在體內(nèi)表達(dá)外源基因時間長等特點(diǎn)，被視為有前途的基因轉(zhuǎn)移載體之一，在基因治療和疫苗研究中得到廣泛應(yīng)用。AAV已被用于多例臨床試驗(yàn)（例如FIX，CFTR，Parkinson's，Canavan疾病）沒有表現(xiàn)出嚴(yán)重的載體相關(guān)不良反應(yīng)。
腺相關(guān)病毒（AAV）抗體：
北京安必奇生物提供不同亞型的腺相關(guān)病毒（AAV）抗體，可特異性識別病毒衣殼；腺相關(guān)病毒亞基或AAV復(fù)制酶。
| 產(chǎn)品編號 | 產(chǎn)品名 | 產(chǎn)品描述 |
| DMAB6351 | Anti-AAV1 (intact particle) monoclonal antibody, Clone BEL2b [Biotin] | Specifically detects AAV1 intact virus particles, both empty and full capsids. DMAB6351 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence, a weak cross-reactivity was observed with AAV6. No reaction with AAV type 2, 3, 4, 5. |
| DMAB6350 | Anti-AAV1 (intact particle) monoclonal antibody, Clone BEL2b | Specifically detects AAV1 intact virus particles, both empty and full capsids. DMAB6350 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence and dot plot analysis, a weak cross-reactivity was observed with AAV6 and AAV12. No reaction with other AAV types. |
| CABT-B9061 | Anti-AAV2 (intact particle) monoclonal antibody, clone B31 [Biotin] | Specifically detects intact virus particles, both empty and full capsids. With AAV2 and AAV3 CABT-B9061 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not for WB. |
| CABT-B9062 | Anti-AAV2 (intact particle) monoclonal antibody, clone B31 | Specifically detects intact virus particles, both empty and full capsids. With AAV2 and AAV3 CABT-B9061 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not for WB. |
| DMAB6346 | Anti-AAV2 Replicase monoclonal antibody, Clone A227.8 | Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in AAV-infected cells. In Immunoblotting prominant reaction with Rep 78 and Rep 68, only faint reaction with Rep 52 and Rep 40. |
| DMAB6345 | Anti-AAV2 Replicase monoclonal antibody, Clone A304.10 | Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells. Cross-reactivity with AAV serotypes 1, 3, 4, 5, 6, 7, 8, 9 and 10 (4,11 and 12 were negative). A weak cross-reactivity is seen in some human cell lines. |
| DMAB6347 | Anti-AAV2 Replicase monoclonal antibody, Clone A77.4 | Recognizes AAV replicase proteins Rep78 and Rep52 in human AAV2-infected cells, no reaction with Rep68 and Rep40. Weak cross-reaction with AAV-serotype 3. |
| DMAB6348 | Anti-AAV2 VP1 monoclonal antibody, Clone B2 | DMAB6348 reacts with VP1 of AAV-2; weak cross-reaction with serotypes 1, 3, 4, 5, 6. In immunoprecipitation, an occasional reaction with a non-AAV-derived protein is found. |
| DMAB6349 | Anti-AAV2 VP1/VP2 monoclonal antibody, Clone B610 | Recognizes AAV-2 viral capsid proteins VP1 and VP2, which are highly enriched in the nucleus. Weak cross-reaction with serogroups 1, 3, and 6. |
| DPAB2423 | Anti-AAV2 VP1/VP2/VP3 polyclonal antibody | Reacts with VP1, VP2 and VP3. Raised against recombinant AAV-2 capsid proteins. |
| CABT-B9063 | Anti-AAV4 (intact particle) monoclonal antibody, clone BEL5 [Biotin] | Specifically detects AAV4 intact virus particles, both empty and full capsids. CABT-B9063 recognizes a conformational epitope of assembled capsids not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. |
| DMAB6352 | Anti-AAV4 (intact particle) monoclonal antibody, Clone BEL5 | Specifically detects AAV4 intact virus particles, both empty and full capsids. DMAB6352 recognizes a conformational epitope of assembled capsids not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. |
| DMAB6355 | Anti-AAV5 (intact particle)monoclonal antibody, Clone BEL6b [Biotin] | Specifically detects intact virus particles, both empty and full capsids. DMAB6355 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting. |
| DMAB6353 | Anti-AAV5 (intact particle) monoclonal antibody, Clone BEL6b | Specifically detects intact virus particles, both empty and full capsids. DMAB6353 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting. |
| DMAB6354 | Anti-AAV5 (intact particle)monoclonal antibody, Clone BEL6c | Specifically detects intact virus particles, both empty and full capsids. DMAB6353 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting. |
| DPAB2424 | Anti-AAV5 polyclonal antiserum | Raised against recombinant AAV5 capsid proteins. The antibody reacts with virus capsid proteins VP1, VP2, VP3 of AAV5; no cross-reaction with AAV types 1 - 4. |
| CABT-B9064 | Anti-AAV6 (intact particle) monoclonal antibody, clone BEL7 | Useful for analysis of the AAV assembly process. CABT-B9064 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Specific for AAV-6. No cross-reaction with AAV type 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, and 12 was observed. Not for use in WB. |
| CABT-B9065 | Anti-AAV8 (intact particle) monoclonal antibody, clone BEL9 | For characterization of different stages of infection and very useful for analysis of the AAV assembly process. CABT-B9065 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in immunoblotting. |
| CABT-B9066 | Anti-AAV8/9 (intact particle) monoclonal antibody, clone BEL9/0 | For characterization of different stages of infection and very useful for analysis of the AAV assembly process. CABT-B9066 specificallyreacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. The antibody cannot be used for immunoblotting using denaturing conditions. |
| DPAB-AV01 | Anti-AAV9 (intact particles)monoclonal antibody, clone BEL0 | Useful for analysis of the AAV assembly process. DPAB-AV01 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in WB. |
腺相關(guān)病毒（AAV）ELISA滴度檢測試劑盒：
檢測AAV滴度的方法目前包括ELISA，qPCR，ddPCR，DNA斑點(diǎn)印跡，AAV轉(zhuǎn)染檢測（Transduction Assay），ICA（Infectious Center Assay）法測感染滴度。
北京安必奇生物可提供不同AAV血清型的ELISA滴度檢測試劑盒。該ELISA試劑盒的捕獲抗體經(jīng)過設(shè)計，可識別野生型、重組型或者組裝的空AAV質(zhì)粒，而不會識別未組裝的AAV蛋白，因此可重復(fù)性高。適合用于rAAV特征鑒定和制備方法優(yōu)化。
| 產(chǎn)品編號 | 產(chǎn)品名 | 規(guī)格 |
| DEIA590 | AAV1 Titration ELISA Kit | 96 Tests |
| DEIA589 | AAV2 Titration ELISA Kit | 96 Tests |
| DEIA591 | AAV5 Titration ELISA Kit | 96 Tests |
| DEIAAV6 | AAV6 Titration ELISA Kit | 96 Tests |
| DEIAAV8 | AAV8 Titration ELISA Kit | 96 Tests |
| DEIAAV9 | AAV9 Titration ELISA Kit | 96 Tests |
